Sequence of the spike protein of the porcine epidemic diarrhoea virus.
Identifieur interne : 000410 ( France/Analysis ); précédent : 000409; suivant : 000411Sequence of the spike protein of the porcine epidemic diarrhoea virus.
Auteurs : M. Duarte [France] ; H. LaudeSource :
- The Journal of general virology [ 0022-1317 ] ; 1994.
Descripteurs français
- KwdFr :
- ADN complémentaire (génétique), Animaux, Clonage moléculaire, Coronaviridae (), Coronaviridae (génétique), Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (génétique), Similitude de séquences d'acides aminés, Suidae, Séquence d'acides aminés, Séquence nucléotidique, Virus de la gastroentérite transmissible (génétique).
- MESH :
- génétique : ADN complémentaire, Coronaviridae, Protéines de l'enveloppe virale, Virus de la gastroentérite transmissible.
- Animaux, Clonage moléculaire, Coronaviridae, Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Similitude de séquences d'acides aminés, Suidae, Séquence d'acides aminés, Séquence nucléotidique.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, Coronaviridae (classification), Coronaviridae (genetics), DNA, Complementary (genetics), Membrane Glycoproteins, Molecular Sequence Data, Sequence Homology, Amino Acid, Spike Glycoprotein, Coronavirus, Swine, Transmissible gastroenteritis virus (genetics), Viral Envelope Proteins (classification), Viral Envelope Proteins (genetics).
- MESH :
- chemical , classification : Viral Envelope Proteins.
- chemical , genetics : DNA, Complementary, Viral Envelope Proteins.
- classification : Coronaviridae.
- genetics : Coronaviridae, Transmissible gastroenteritis virus.
- Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, Membrane Glycoproteins, Molecular Sequence Data, Sequence Homology, Amino Acid, Spike Glycoprotein, Coronavirus, Swine.
Abstract
The complete sequence of the spike (S) gene of the Br1/87 isolate of porcine epidemic diarrhoea virus (PEDV) was determined from cDNA clones. The predicted polypeptide was 1383 amino acids long, contained 29 potential N-linked glycosylation sites and showed structural features similar to those of the coronavirus spike protein. The PEDV S protein, like that of the members of the transmissible gastroenteritis virus (TGEV)-related subset, lacks a proteolytic site to yield cleaved amino and carboxy subunits S1 and S2. Viral polypeptide species of the expected M(r), i.e. 170K/190K, were observed in PEDV-infected cells. Sequence comparison confirmed that, within the subset, PEDV was most closely related to the human respiratory coronavirus HCV 229E. However, PEDV S protein has an additional 250 residue N-terminal domain which is absent from HCV 229E and porcine respiratory coronavirus, the respiratory variant of TGEV. Alignment of the S1 regions revealed a second domain of about 90 residues with increased sequence divergence which might possibly express virus-specific determinants.
DOI: 10.1099/0022-1317-75-5-1195
PubMed: 8176382
Affiliations:
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pubmed:8176382Le document en format XML
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Duarte</name><affiliation wicri:level="1"><nlm:affiliation>I.N.R.A., Unité de Virologie et Immunologie Moléculaires, CR de Jouy-en-Josas, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>I.N.R.A., Unité de Virologie et Immunologie Moléculaires, CR de Jouy-en-Josas</wicri:regionArea><wicri:noRegion>CR de Jouy-en-Josas</wicri:noRegion><wicri:noRegion>CR de Jouy-en-Josas</wicri:noRegion></affiliation></author><author><name sortKey="Laude, H" sort="Laude, H" uniqKey="Laude H" first="H" last="Laude">H. Laude</name></author></analytic><series><title level="j">The Journal of general virology</title><idno type="ISSN">0022-1317</idno><imprint><date when="1994" type="published">1994</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Cloning, Molecular</term><term>Coronaviridae (classification)</term><term>Coronaviridae (genetics)</term><term>DNA, Complementary (genetics)</term><term>Membrane Glycoproteins</term><term>Molecular Sequence Data</term><term>Sequence Homology, Amino Acid</term><term>Spike Glycoprotein, Coronavirus</term><term>Swine</term><term>Transmissible gastroenteritis virus (genetics)</term><term>Viral Envelope Proteins (classification)</term><term>Viral Envelope Proteins (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (génétique)</term><term>Animaux</term><term>Clonage moléculaire</term><term>Coronaviridae ()</term><term>Coronaviridae (génétique)</term><term>Données de séquences moléculaires</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale ()</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Similitude de séquences d'acides aminés</term><term>Suidae</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Virus de la gastroentérite transmissible (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="classification" xml:lang="en"><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Complementary</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Coronaviridae</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronaviridae</term><term>Transmissible gastroenteritis virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN complémentaire</term><term>Coronaviridae</term><term>Protéines de l'enveloppe virale</term><term>Virus de la gastroentérite transmissible</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Cloning, Molecular</term><term>Membrane Glycoproteins</term><term>Molecular Sequence Data</term><term>Sequence Homology, Amino Acid</term><term>Spike Glycoprotein, Coronavirus</term><term>Swine</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Clonage moléculaire</term><term>Coronaviridae</term><term>Données de séquences moléculaires</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Similitude de séquences d'acides aminés</term><term>Suidae</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The complete sequence of the spike (S) gene of the Br1/87 isolate of porcine epidemic diarrhoea virus (PEDV) was determined from cDNA clones. The predicted polypeptide was 1383 amino acids long, contained 29 potential N-linked glycosylation sites and showed structural features similar to those of the coronavirus spike protein. The PEDV S protein, like that of the members of the transmissible gastroenteritis virus (TGEV)-related subset, lacks a proteolytic site to yield cleaved amino and carboxy subunits S1 and S2. Viral polypeptide species of the expected M(r), i.e. 170K/190K, were observed in PEDV-infected cells. Sequence comparison confirmed that, within the subset, PEDV was most closely related to the human respiratory coronavirus HCV 229E. However, PEDV S protein has an additional 250 residue N-terminal domain which is absent from HCV 229E and porcine respiratory coronavirus, the respiratory variant of TGEV. Alignment of the S1 regions revealed a second domain of about 90 residues with increased sequence divergence which might possibly express virus-specific determinants.</div></front></TEI><affiliations><list><country><li>France</li></country></list><tree><noCountry><name sortKey="Laude, H" sort="Laude, H" uniqKey="Laude H" first="H" last="Laude">H. Laude</name></noCountry><country name="France"><noRegion><name sortKey="Duarte, M" sort="Duarte, M" uniqKey="Duarte M" first="M" last="Duarte">M. Duarte</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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